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CABRI:NCCB_PHAGES |
Collection_number |
NCCB 3079
|
Name |
lambda gt11
|
Type |
phage
|
Other_collection_numbers |
PC-V3079
|
Restricted_distribution |
No
|
Literature |
Young, R.A. et al., Proc. Natl. Acad. Sci. USA (1983) 80:1194-1198 [PMID: 6219389
] |
History_of_deposit |
-
|
Host_for_propagation |
Y1088 = PC 2944
|
Host_used_for_propagation |
-
|
Lysogenicity |
Yes
|
Virus_used_for |
-
|
Cell_surface_receptor |
LamB
|
Medium |
Medium 171
|
Class |
Recombinant
|
Host_for_selection |
Y1090 = PC 2933 (or Y1090 r- = PC 2941) (lacZ-, su
|
Plaque_forming_phage |
No
|
Clear_plaque_morphology |
No
|
Minimum_packaging_capacity |
0 kb
|
Maximum_packaging_capacity |
7.2 kb
|
Nucleic_acid_type |
DNA,double stranded,linear
|
Further_information |
preparation of lysogens on Y1089 = PC 2942 (see Sambrook, J., Fritsch, E.F. and
Maniatis, T., Molecular Biology, a laboratory manual, CSH, (1989), chapter 12 (chapter 2 for
growth and properties); recognition of recombinants: lac- phenotype on lacZ- host; properties of
recombinants: cIts, Gam+, Int+, Red+ lambda insertion vector
|
Restriction_sites |
(U,E,I(lacZ)):EcoRI
|
Price_code |
1
|
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